APC Anti-Mouse/Human Ki-67

Product Details


CloneK67AR2b
ApplicationFC
ReactivityHuman, Mouse
FormatAPC
Target NameKi-67, Ki67
IsotypeRat IgG2b
Antibody TypeMonoclonal
Regulatory StatusRUO
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
Protein ConcentrationSupplied at a lot-specific concentration.
Storage&HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Recommended UsageFor flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application.
Excitation LaserRed Laser (633 nm)

Background Information


Ki-67 is a nuclear protein expressed as two isoforms (395 and 345 kDa), each containing a forkhead-associated (FHA) domain and 16 Ki-67 repeats that include the epitope recognized by the Ki-67 monoclonal antibody. Ki-67 interacts with proteins such as Hklp2, hNIFK, and chromobox homologs 1, 3, and 5. Its expression is restricted to proliferating cells in the G1, S, G2, and M phases of the cell cycle, making it a robust marker of cell proliferation. Ki-67 is widely used in oncology as a prognostic factor, in studies of lymphocyte and myocyte proliferation, and as a marker of neuronal proliferation in various neuropathologies.

Data Sheets


APC Anti-Mouse/Human Ki-67 TDS

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