| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 810403 | 25 ug | $245 | ||
| 810404 | 100 ug | $595 |
| Application | ELISA, BLI |
|---|---|
| Format | Liquid, Biotinylated |
| Expression Host | CHO |
| Target Name | CD3-epsilon, FLJ18683, T3E, TCRE, CD3E |
| Species | Human |
| Sources | Recombinant Human CD3 Protein ( Asp 23- Asp126) with C-terminus Fc-Avi-tag is expressed in CHO cell. This protein was site-specifically labeled with Biotin by BirA ligase. |
| Accession Number | P07766 |
| Molecular Weight | The protein has a predicted molecular weight of 40.2 kDa. Under DTT-reducing conditions, it migrates at approximately 50 kDa on SDS-PAGE . |
| Affinity Tag | C-Fc-Avi |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 1xPBS buffer, pH7.4, 0.22 µm filtered |
| Endotoxin level | Less than 0.1 EU/µg protein as determined by the LAL method |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
CD3ε is a critical component of the T cell receptor (TCR)-CD3 complex, a transmembrane receptor essential for antigen recognition and T cell activation. It is a single-pass type I membrane glycoprotein containing an Ig-like extracellular domain and a cytoplasmic ITAM motif, which initiates intracellular signaling upon phosphorylation by Src family kinases (e.g., LCK, FYN). The TCR-CD3 complex includes two CD3ε chains, CD3γ, CD3δ, CD3ζ, and a TCR α/β or γ/δ heterodimer. CD3ε is expressed on all mature T cells, NK T cells, and some thymocytes, playing a vital role in T cell development and immune response. Mutations in CD3E disrupt T cell maturation and can lead to severe combined immunodeficiency (SCID), as well as increased susceptibility to autoimmune diseases such as type I diabetes.
Biotinylated Human CD3 (C-Fc-Avi) TDS
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