| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 602203 | 25 ug | $245 | ||
| 602204 | 100 ug | $595 |
| Application | ELISA, BLI |
|---|---|
| Format | Liquid, Biotinylated |
| Expression Host | CHO |
| Target Name | TIM3, HAVCR2, TIMD3, FLJ14428, KIM3 |
| Species | Human |
| Accession Number | Q8TDQ0 |
| Sources | Recombinant human Tim-3 protein (Ser22-Arg200) with C-terminus His-Avi tag was expressed in CHO Cells. This protein was site-specifically labeled with Biotin by BirA ligase. |
| Molecular Weight | This protein has a predicted molecular weight of 23.7 kDa. Under DTT-reducing conditions, the protein migrates at approximately 45 kDa on SDS-PAGE. |
| Affinity Tag | C-His-Avi |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 1xPBS buffer, pH7.4, 0.22 µm filtered |
| Endotoxin level | Not tested |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
HAVCR2, also known as TIM-3, is a transmembrane glycoprotein predominantly expressed on terminally differentiated Th1 cells and various activated immune cells such as CD8+ T cells, NK cells, dendritic cells, and macrophages. It contains an IgV-like domain and a Ser/Thr-rich mucin region. TIM-3 interacts with its ligand Galectin-9 to negatively regulate IFN-γ production, promote immune tolerance, and suppress Th1-mediated autoimmune responses. Dysregulation of the TIM-3/Galectin-9 pathway is implicated in chronic autoimmune diseases like multiple sclerosis and systemic lupus erythematosus. TIM-3 also plays an immunosuppressive role in cancer by inhibiting antitumor cytotoxic T cell activity and reducing tumor-infiltrating lymphocytes. The soluble form of TIM-3 (sTIM-3) further impairs T cell-mediated immunity, highlighting its potential as a therapeutic target in autoimmune diseases and cancer.
Biotinylated Human Tim-3 Protein (C-His-Avi) TDS
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