| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 201811 | 25 tests | $60 | ||
| 201812 | 100 tests | $150 |
| Clone | 2.4G2 |
|---|---|
| Application | FC |
| Reactivity | Mouse |
| Format | FITC |
| Target Name | CD16/32 |
| Isotype | Rat IgG2b |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage&Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. |
| Excitation Laser | Blue Laser (488 nm) |
CD16/32 are Fc-gamma receptors (FcγRs) expressed on a variety of immune cells, including B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD16 corresponds to the low-affinity Fc receptor III (FcγRIII), while CD32 corresponds to Fc receptor II (FcγRII). These receptors bind antibody-antigen immune complexes, linking innate and adaptive immunity and mediating adaptive immune responses. In research, antibodies against CD16/CD32 are commonly used to block Fc receptor-mediated interactions, preventing non-specific binding of antibodies or immunoglobulin complexes to immune cells during experiments such as flow cytometry and immunohistochemistry, thereby improving experimental accuracy.
FITC Rat IgG2b Isotype control
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