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HRP Labeled Human ACE-2 Protein (N-His)

SARS-CoV-2 Spike RBD Protein (C-Fc) (Catalog #602501) is coated at 2 µg/mL (200 ng/well). HRP-labeled human ACE2 (N-His) demonstrates dose-dependent binding to the SARS-CoV-2 Spike RBD (C-Fc), as confirmed by quality testing. The ED₅₀ is approximately 5–20 ng/mL.

SARS-CoV-2 Spike S1 protein (Catalog #603101) is coated at 2 µg/mL (200 ng/well). HRP-labeled human ACE2 (N-His) demonstrates dose-dependent binding to the SARS-CoV-2 Spike S1 protein, as confirmed by quality testing. The ED₅₀ is approximately 5–20 ng/mL.

SARS-CoV-2 Spike Trimer protein (Catalog # 603701) is coated at 2 µg/mL (200 ng/well). HRP-labeled human ACE2 (N-His) demonstrates dose-dependent binding to the SARS-CoV-2 Spike Trimer protein, as confirmed by quality testing. The ED₅₀ is approximately 5–20 ng/mL.

Cat #	Size	Price	Quantity
600005	25 ug	$245
600006	100 ug	$595

Bulk/Custom

Product Details

Application	ELISA
Format	Liquid, HRP conjugated
Expression Host	HEK293
Target Name	ACE2, ACE-2, Angiotensin I converting Enzyme 2, ACEH
Species	Human
Accession Number	Q9BYF1
Sources	Human ACE2 protein (Gln18-Ser740) with N-terminus His tag is expressed in HEK293 cells and conjugated to HRP.
Molecular Weight	This protein has a predicted molecular weight of 86.5 kDa. Under DTT-reducing conditions, the protein migrates at approximately 90-110 kDa on SDS-PAGE.
Affinity Tag	N-His
Regulatory Status	RUO
Formulation	1xPBS buffer, pH7.4, 0.22 µm filtered, with 50% Stabilizer
Endotoxin level	Not tested
Protein Concentration	25µg size is bottled at 0.1mg/mL concentration. 100 µg size is supplied at a lot-specific concentration.
Storage and Handling	Quick spin the vial after receiving. Unopened vial can be stored at 4°C for six months.

Background Information

Angiotensin-converting enzyme 2 (ACE2) is a type I transmembrane zinc metalloprotease with about 60% homology to ACE, consisting of 805 amino acids including a signal peptide, catalytic domain, membrane anchor, and cytoplasmic tail. ACE2 regulates the renin-angiotensin system by converting angiotensin I to angiotensin 1-9 and angiotensin II to the vasodilator angiotensin 1-7, thus counteracting vasoconstriction and playing a key role in cardiovascular and renal function. ACE2 also serves as the functional receptor for human coronaviruses SARS-CoV, SARS-CoV-2, and HCoV-NL63, facilitating viral entry. Its expression is high in lung, heart, kidney, intestine, testis, and vascular cells. Beyond cardiovascular regulation, ACE2 is involved in respiratory disease pathogenesis, including acute respiratory distress syndrome, and has been linked to diabetes and hypertension. Additionally, its homolog collectrin is implicated in amino acid transport and genetic disorders like Hartnup disease.

Data Sheets

HRP Labeled Human ACE-2 Protein (N-His) TDS

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