| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 814001 | 25 ug | $145 | ||
| 814002 | 100 ug | $295 |
| Application | ELISA, BLI |
|---|---|
| Format | Liquid, Purified |
| Expression Host | CHO |
| Target Name | B7-H5, SISP1, Gi24, VISTA |
| Species | Human |
| Sources | Recombinant Human Vista (Phe33-Ala194) with C-terminus Fc-Avi-tag is expressed in CHO cell. |
| Accession Number | Q9H7M9 |
| Molecular Weight | The protein has a predicted molecular weight of 47 kDa. Under DTT-reducing conditions, it migrates at approximately 55-65 kDa on SDS-PAGE. |
| Affinity Tag | C-Fc-Avi |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 1xPBS buffer, pH7.4, 0.22 µm filtered |
| Endotoxin level | Not tested |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
VISTA (V-domain Ig suppressor of T cell activation), also known as VSIR, PD-1H, or C10orf54, is a type I transmembrane protein with a single IgV-like extracellular domain and functions as an inhibitory immune checkpoint molecule. It is broadly expressed on myeloid cells, T cells, dendritic cells, and in tissues such as spleen and bone marrow. VISTA suppresses CD4⁺ and CD8⁺ T cell activation and cytokine production, contributing to immune tolerance and regulation. It may also play a role in embryonic stem cell differentiation by modulating BMP4 signaling. VISTA undergoes proteolytic cleavage, generating both soluble and membrane-bound fragments, and its interaction with PSGL1 in low-pH tumor microenvironments has been reported. Due to its immunosuppressive properties, VISTA is considered a promising target for cancer immunotherapy and may be involved in inflammatory conditions such as chronic rhinosinusitis with nasal polyps.
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