| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 109405 | 25 tests | $30 | ||
| 109406 | 100 tests | $80 |
| Clone | HI100 |
|---|---|
| Application | FC |
| Reactivity | Human |
| Format | PE |
| Target Name | CD45RA, GP180, LY5, LCA, PTPRC |
| Isotype | Mouse IgG2b |
| Antibody Type | Monoclonal |
| Regulatory Status | RUO |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA |
| Protein Concentration | Supplied at a lot-specific concentration. |
| Storage and Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Recommended Usage | For flow cytometric staining, it is recommended to use 5 uL of this reagent per 0.5-1.0 million cells in a 100 µL volume. Optimal reagent performance should be determined by titration for each specific application. |
| Excitation Laser | Blue Laser (488 nm) Green/Yellow laser (532/561nm) |
CD45RA is a 205–220 kDa isoform of the CD45 tyrosine phosphatase, expressed on naïve/resting T cells, medullary thymocytes, B cells, and monocytes. It is found on ~40–50% of peripheral CD4⁺ T cells, ~50% of CD8⁺ T cells, and subsets of B cells and monocytes. CD45RA enhances TCR and BCR signaling and associates with molecules like LPAP, CD1–CD4, and galectin-1. Its expression is regulated by cytokines and can be present on some activated T cells. CD45RA-specific antibodies (e.g., HI100) are widely used to identify naïve T cells and study CD4⁺ suppressor/inducer subsets.
PE Mouse IgG2b Isotype Control
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