| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 604101 | 25 ug | $145 | ||
| 604102 | 100 ug | $325 |
| Application | Enzymatic reaction |
|---|---|
| Format | Liquid, Purified |
| Expression Host | E.coli |
| Target Name | BirA ligase |
| Species | Escherichia coli |
| Accession Number | P06709 |
| Sources | BirA ligase with N-terminus GST is expressed in E.coli cells. |
| Molecular Weight | This protein has a predicted molecular weight of 62.2 kDa. Under DTT-reducing conditions, the protein migrates at approximately 65 kDa on SDS-PAGE. |
| Affinity Tag | N-GST |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 20mM Tris, 300mM NaCL, 5mM DTT, 10% glycerol |
| Endotoxin level | Not tested |
| Protein Concentration | 25µg size is bottled at 0.3mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm filtered Tris PH 7.5 buffer. For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
The BirA ligase is a key reagent because of its ability to biotinylate the target protein at specific site
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