| Cat # | Size | Price | Quantity | |
|---|---|---|---|---|
| 606401 | 25 ug | $145 | ||
| 606402 | 100 ug | $295 |
| Application | ELISA, BLI |
|---|---|
| Format | Liquid, Purified |
| Expression Host | CHO |
| Target Name | TNFSF9, 4-1BB Ligand, CD137L |
| Species | Human |
| Accession Number | P41273 |
| Sources | Recombinant Human TNFSF15 (Leu72-Leu251) with N-terminus His tag is expressed in CHO cells. |
| Molecular Weight | This protein has a predicted molecular weight of 21.6 kDa. Under DTT-reducing conditions, the protein migrates at approximately 23 kDa on SDS-PAGE. |
| Affinity Tag | N-His |
| Purity | >95% based on SDS-PAGE under reducing condition |
| Regulatory Status | RUO |
| Formulation | 1xPBS buffer, pH7.4, 0.22 µm filtered |
| Endotoxin level | Less than 0.1 EU/µg protein as determined by the LAL method |
| Protein Concentration | 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is supplied at a lot-specific concentration. |
| Storage and Handling | Briefly centrifuge the vial upon receipt. An unopened vial can be stored at 4°C for up to 2 weeks, or at -20°C or below for up to six months. The protein may be further diluted to 0.1 mg/mL using 0.22 µm-filtered PBS buffer (pH 7.4). For long-term storage, the diluted stock solution should be aliquoted and stored at ≤ –70°C to minimize freeze-thaw cycles. If additional dilution is required, carrier proteins such as FBS or BSA should be added to maintain protein stability. |
4-1BB Ligand (4-1BBL), also known as CD137L, is a 38 kDa type II transmembrane protein in the TNF superfamily, composed of a cytoplasmic domain, a transmembrane segment, and a large extracellular domain. It is expressed on activated immune cells including B cells, monocytes, macrophages, dendritic cells, T cells, as well as lymphoma/myeloma cells, hematopoietic progenitors, neurons, and astrocytes. A soluble 26 kDa form is also bioactive. 4-1BBL binds to the receptor 4-1BB (CD137/TNFRSF9) on activated CD4+ and CD8+ T cells, NK cells, and various myeloid cells, delivering co-stimulatory signals that promote T cell proliferation, activation, and survival, particularly during later stages of immune responses and memory T cell maintenance. Reverse signaling through 4-1BBL on monocytes and macrophages induces inflammatory cytokine production and modulates immune cell development.
Human 4-1BBL/TNFSF9 Protein (N-His) TDS
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